The VADEX technology is a self-adjuvanting protein nanoparticle platform. The building block of VADEX technology is a fusion protein combining an amphipathic helical peptide (AHP) and a superfolder GFP (sfGFP). The precisely designed 19 a.a. AHP serves both as a polymerization module and an adjuvant. This fusion protein can be self-assembled into a thermally-stable protein nanoparticle (PNP) that endures ambient temperature storage up to 40 oC for two months. A target antigen can be expressed on the surface of nanoparticle by either loop insertion (VADEX-PEP) or strand complementation (VADEX-Pro) through sfGFP. Being self-adjuvanting, VADEX can truly enables Global Vaccination effort that protects every child from Sahara desert to Amazon rain forest.