The VADEX technology is a self-adjuvanting protein nanoparticle platform. The building block of VADEX technology is a fusion protein combining an amphipathic helical peptide (AHP) and a superfolder GFP (sfGFP). The precisely designed 19 a.a. AHP serves both as a polymerization module and an adjuvant. This fusion protein can be self-assembled into a thermally-stable protein nanoparticle (PNP) that endures ambient temperature storage up to 40 oC for two months. A target antigen can be expressed on the surface of nanoparticle by either loop insertion (VADEX-PEP) or strand complementation (VADEX-Pro) through sfGFP. Being self-adjuvanting, VADEX can truly enables Global Vaccination effort that protects every child from Sahara desert to Amazon rain forest.
Applications: Target peptide with a size less than 90 amino acids can be inserted through loop insertion into the loop9 of sfGFP to form a fusion protein. The fusion protein will self-assemble into protein nanoparticle after protein expression in bacteria and used as antigen for stimulate long-lasting humoral immune response.
Publication: Wong T-T, Liou G-G, Kan M-C. A Thermal-Stable Protein Nanoparticle That Stimulates Long Lasting Humoral Immune Response. Vaccines. 2023; 11(2):426. https://doi.org/10.3390/vaccines1102042
Applications: Target protein of any size can be fused with the strand-11 of sfGFP and be incorporated onto the protein nanoparticle when co-expressed with AH-sfGFP-10 fusion protein in bacteria. The purified protein nanoparticle can be used as an antigen for stimulating the induction of IgG with 100 folds higher affinity than antigen with AddaVax.
To facilitate the research of self-adjuvanted protein nanoparticle, VADEX, free samples of VADEX-Pep and VADEX-Pro protein nanoparticles are available with shipping/handle charge of 100 USD, please place your request by contacting Dr. Ming-Chung Kan
email: mingchung.kan@vaxsia.com
VADEX-Pep: LYRRLE-sfGFP-2xhM2e
VADEX-Pro: MBP-C